|Mumps Virus PCR
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|Nucleic acid amplification by PCR to detect Mumps virus RNA
|Avg. Turnaround Time
|Coralville, 8 a.m. - 5 p.m., M-F
|1 - 3 business days
|Nucleic Acid Amplification by Polymerase Chain Reaction (PCR)
|Buccal/Oral swab. PCR is a very sensitive test, and precautions should be taken to not cross-contaminate specimens (i.e., wear gloves and change before and after collecting specimen. Avoid contact with environmental surfaces).
|Collect specimen using a SHL Virus Isolation and Detection Kit (contains M4-RT viral transport medium and swabs) or equivalent viral transport medium.
Buccal (Oral) Swab: The buccal cavity is the space between the cheek and teeth. The parotid duct drains in the space near the upper molars. Massage the parotid gland area just in front of the ear and near the angle of the jaw for 30 seconds prior to collecting secretions on the swab. Swab the buccal cavity by sweeping the swab near the upper molar to the lower molar. Place swab in M4-RT viral transport medium and do not remove swab.
|Temperature and Stability:
|Refrigerate (2-8°C); DO NOT FREEZE; Overnight
|Frozen samples; Sample obtained on cotton-tipped, wooden-shafted or calcium alginate swabs.
|Not Detected, Detected, Indeterminate, Equivocal
|Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
|Presence of Mumps virus RNA is indicative of mumps infection.