Click for the 2015-2016 Testing Algorithm

Hygienic Laboratory Influenza Surveillance Testing

For the 2015-2016 season, SHL and the Iowa Department of Public Health requests the following specimens to be submitted for molecular testing at SHL:

I. Influenza Specimen Collection, Submission and Results

Please submit the patient’s specimen to SHL for testing by real-time PCR (RT-PCR) if it meets the criteria as described in the algorithm. RT-PCR is a molecular method of virus detection that is more sensitive and specific than point of care rapid tests.

Specimen Collection

Patients with influenza-like illness (optimally within 3 days of symptom onset):

Patients hospitalized with pneumonia or other secondary infections when more than seven days has elapsed from the onset of influenza-like illness:

The following specimens should be considered for patients who are intubated or undergoing bronchoscopy:

If a patient with pneumonia or other secondary infections is not intubated or undergoing bronchoscopy, then a nasopharyngeal swab or combined nasal swab plus throat swab (2 swabs in one tube transport medium) should be obtained.

Test Request Form

II. Transportation of specimens to SHL

III. Accessing Your Results

IV. Result Interpretation

All testing is performed using the CDC Human Influenza Virus Real–Time RT–PCR Diagnostic Panel and the results are reported as follows:

If a specimen is positive for Influenza A but negative for all subtypes it is considered inconclusive. This is most likely due to a weakly positive sample that does not contain sufficient amounts of virus for subtyping, but could also reflect an infection with a novel strain of influenza virus. Any of these unusual results are investigated by SHL.

Influenza surveillance serves the following purposes:

Contact Us:

  • Jeff Benfer, Virology and Molecular Biology Supervisor, 319-335-4276
  • Lucy E. DesJardin, PhD, Program Manager for Molecular Research and Development, 319-335-4339
  • Last updated Sept. 18, 2015.